Subject Code & Name: EL25404 – Instrumental Methods of Analysis
Regulation: R-2025
Semester: IV (Fourth Semester)
Branch: B. Tech. Chemical and Electrochemical Engineering (ChemElec)
Credits / L-T-P: 3 Credits | L-T-P: 3-0-0
Course Objectives
- To enable students to understand the fundamentals and mechanisms of spectral analysis, qualitative and quantitative spectroscopic techniques, IR spectroscopy, and chromatographic methods used in chemical analysis.
Full Unit-wise Syllabus
Unit I – Introduction To Spectroscopical Methods of Analysis
Electromagnetic radiation: various ranges, dual properties, various energy levels, interaction of photons with matter, absorbance & transmittance and their relationship, permitted energy levels for the electrons of an atom and simple molecules, various electronic transitions in organic and inorganic compounds effected by UV, and visible radiations, various energy level diagrams of saturated, unsaturated and carbonyl compounds, excitation by UV and visible radiations, choice of solvents, cut off wavelengths for solvents.
Activities: Numerical problems on absorbance and transmittance; interpretation of energy level diagrams.
Unit II – Qualitative Analysis by UV And Visible Spectrocopy
Lamda max and epsilon max rules, Woodward -Fieser rules for the calculation of absorption maxima (Lamda max) for dienes and carbonyl compounds, Effects of auxochromes and effects of conjugation on the absorption maxima, Different shifts of absorption peaks (Bathochromic, hypsochromic, hypochromic), Instrumentation for UV and Visible spectrophotometers (source, optical parts and detectors), Applications of UV and Visible spectroscopy.
Activities: Prediction of λmax using Woodward–Fieser rules; interpretation of UV spectra.
Unit III – Quantitative analysis By UV And Visible Spectrocopy
Beer-Lambert's law, limitations, deviations (real, chemical, instrumental), estimation of inorganic ions such as Fe, Ni and estimation of nitrite using Beer -Lambert's law, multicomponent analysis (no overlap, single way overlap and two-way overlap), photometric titration (experimental set -up and various types of titrations and their corresponding curves).
Activities: Numerical problems based on Beer–Lambert law; analysis of photometric titration curves.
Unit IV – IR Spectroscopy
Theory of IR spectroscopy, various stretching and vibration modes for diatomic and triatomic molecules (both linear and nonlinear), various ranges of IR (near, mid, finger print and far) and their usefulness, Instrumentation (only the sources and detectors used in different regions), sample preparation techniques, qualitative analysis of alkanes, alkenes and carbonyl compounds.
Activities: Interpretation of IR spectra; identification of functional groups.
Unit V – Chromatographic Methods
Classification of chromatographic methods, column, thin layer, paper, gas, High Performance Liquid Chromatographical methods (principle, mode of separation and technique).
Activities: Separation case studies; analysis of chromatograms and retention times.
Course Outcomes (COs)
- CO1: Understand the principles, instrumentation, and applications of spectroscopic, chromatographic, and electroanalytical techniques used in chemical analysis.
- CO2: Apply spectroscopic, chromatographic, and electroanalytical methods for qualitative and quantitative chemical analysis.
- CO3: Analyze analytical data obtained from UV-Visible, IR, chromatographic, and electrochemical techniques for chemical characterization.
- CO4: Evaluate the performance and suitability of analytical instruments and methodologies in terms of accuracy, precision, sensitivity, and reliability for industrial applications.
Assessment Pattern (Quick Note)
- Weightage: Continuous Assessment 40% | End Semester Examinations 60%
- Internal methodology: Assignments (20%), Application-oriented problem solving (20%), Research Paper presentation (10%), Internal Examinations (50%)
Source: Official Anna University – B. Tech. Chemical and Electrochemical Engineering R-2025 Syllabus
Last Updated: October 2026
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