PY25305 Biochemistry and Physiology Laboratory – Semester III – Pharma – R-2025

Subject Code & Name: PY25305 – Biochemistry and Physiology Laboratory

Regulation: R-2025

Semester: III (Third Semester)

Branch: B.Tech. Pharmaceutical Technology (Pharma)

Credits / L-T-P: 2 Credits | L-T-P: 0-0-4

Course Objectives

• • To equip students with practical skills in analysing biochemical constituents and physiological parameters, and interpreting results for health assessment and pharmaceutical relevance through hands-on laboratory techniques.

Full Unit-wise Syllabus

List of Experiments

• Section A – Biochemistry
• Qualitative tests for carbohydrates
• Qualitative tests for proteins and amino acids
• Estimation of blood glucose (GOD-POD method)
• Estimation of serum proteins (total protein and albumin)
• Estimation of serum cholesterol and triglycerides
• Preparation and pH measurement of buffers
• Paper chromatography/TLC for amino acid separation
• Enzyme activity assay (e.g., Salivary amylase on starch)
• Enzyme Isolation / Enzyme Encapsulation Section B – Physiology
• Measurement of blood pressure using sphygmomanometer
• Recording of pulse rate and respiratory rate
• Blood smear examination – RBC, WBC morphology, Total and Differential Count
• Determination of hemoglobin content (Sahli’s method)
• Estimation of bleeding time and clotting time
• Blood group determination
• Preparation of physiological solution: Ringers’ solution, Normal saline

Course Outcomes (COs)

• CO1: Understand the principles and procedures involved in basic biochemical identification and estimation techniques.
• CO2: Apply clinical and laboratory methods to assess common physiological parameters and biochemical constituents.
• CO3: Analyze experimental data and correlate biochemical and physiological variations with normal and pathological conditions.
• CO4: Apply laboratory techniques in diagnostic investigations and pharmaceutical applications.
• CO5: Demonstrate safe, accurate, and ethical laboratory practices while handling biological samples and analytical instruments.
• CO6: Evaluate blood, urine, and blood grouping test results and interpret their significance in physiological and pathological conditions. Semester IV.

Source: Official Anna University – B.Tech. Pharmaceutical Technology R-2025 Curriculum
Last Updated: September 2026